In the framework of the Génopole programme,
and with funding support from the
Association Raoul Follereau and the World
Health Organisation, the Institut Pasteur is sequencing
of an epidemic strain of M. ulcerans.
ulcerans is a close relative of M. marinum
and M. tuberculosis.
This mycobacterium is the causative agent of Buruli
ulcer, a severe human skin disease
that occurs worldwide
but predominantly in tropical
regions, most notably in many
countries throughout central
and west Africa. Infection with M.
ulcerans results in chronic and
ulcers, often further complicated by
osteomyelytis. Radical surgical excision of
infected tissue is currently the only effective treatment.
The M. ulcerans genome has been estimated to be
approximately 4.6 Mb in size with a
G+C content of around 65%.
Shotgun sequencing is in progress, and a
database of contigs is avalable for
searching on our BLAST Server.
There are 42,239 reads
giving a theoretical coverage of
99.7% of the genome,
assuming a genome size of 4.6Mb.
Assembly has begun, and the data for the latest
assembly are available. At present, there are a total of
1597 contigs (56%>1kb and 10%>10 kb); a total size of 6.032 Mb.
that BuruList is currently
operating only as a BLAST
Server as the genome is still in
a preliminary stage of
assembly. The assembly database
contains unedited automatic assemblies and it will
contain some errors and missassemblies. The contig numbers in the databases are not stable, and will vary with each data release.